INDUCIBLE GENE TARGETING IN MICE

被引：1531

作者：

KUHN, R ^{[1
]}

SCHWENK, F ^{[1
]}

AGUET, M ^{[1
]}

RAJEWSKY, K ^{[1
]}

机构：

[1] UNIV ZURICH, INST MOLEC BIOL 1, CH-8093 ZURICH, SWITZERLAND

来源：

SCIENCE | 1995年 / 269卷 / 5229期

关键词：

D O I：

10.1126/science.7660125

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase, Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by loxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.

引用

页码：1427 / 1429

页数：3

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