SUBPOPULATION OF HYPERRESPONSIVE POLYMORPHONUCLEAR NEUTROPHILS IN PATIENTS WITH ADULT RESPIRATORY-DISTRESS SYNDROME - ROLE OF CYTOKINE PRODUCTION

To gain further insight into the pathogenesis of the adult respiratory distress syndrome (ARDS), we studied possible relationships among the activation status of circulating polymorphonuclear neutrophils (PMN), cytokine levels, and the severity of lung injury in 31 patients: 15 with ARDS, nine with severe pneumonia uncomplicated by ARDS, and seven mechanically ventilated with neither ARDS nor pneumonia. Nine healthy subjects served as controls. Using flow cytometry, we identified a subpopulation of PMN with an increased capacity to generate hydrogen peroxide after stimulation ex vivo in all three patient groups; significantly higher values were found in those with ARDS. The PMN stimulation index, a reflection of the degree of hyporesponsiveness, correlated with elevated levels of tumor necrosis factor-alpha (TNFalpha) in plasma, and both spontaneous and lipopolysaccharide-induced TNFalpha production by cultured monocytes. These biologic expressions of PMN activation and cytokine generation both correlated with indices of the severity of lung injury, but not with the overall clinical severity. In contrast, IL,6 and IL-1beta showed little or no relationship with either the degree of lung injury or PMN hyporesponsiveness. We conclude that TNFalpha-primed PMN may play a major role in the pathogenesis of ARDS-associated lung injury.