AN ELISA PLATE BASED ASSAY FOR HYALURONAN USING BIOTINYLATED PROTEOGLYCAN-G1 DOMAIN (HA-BINDING REGION)

被引：110

作者：

FOSANG, AJ

HEY, NJ

CARNEY, SL

HARDINGHAM, TE

机构：

[1] KENNEDY INST, DIV BIOCHEM, 6 BUTE GARDENS, LONDON W6 7DW, ENGLAND

[2] LILLY RES CTR LTD, WINDLESHAM GU20 6PH, SURREY, ENGLAND

来源：

MATRIX | 1990年 / 10卷 / 05期

基金：

英国医学研究理事会;

关键词：

HA-binding region; hyaluronan; proteoglycan;

D O I：

10.1016/S0934-8832(11)80186-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme-linked absorbent assay for the determination of hyaluronan (HA) has been developed. The procedure is sensitive, simple and is based on a microtitre plate format. The assay involves competition between HA absorbed to the plate and HA free in solution for binding to biotinylated cartilage proteoglycan binding region (G1 domain). The range of the assay is 10-2500 ng/ml with 50% inhibition at about 200 ng/ml. The assay can be used in guanidine HCl up to 0.6 M and in 0.5 % deoxycholate or 0.5 % nonidet. This new technique involves fewer experimental steps and is simpler to perform than other methods. © 1990, Gustav Fischer Verlag · Stuttgart · New York. All rights reserved.

引用

页码：306 / 313

页数：8

共 36 条

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[2]

BENTLEY JP, 1968, REPAIR REGENERATION, P151

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