IN VITRO METABOLISM OF TESTOSTERONE-4-14C AND DELTA4-ANDROSTENE-3,17-DIONE-4-14C IN HUMAN SKIN

Testosterone-4-14C was incubated with specimens of human skin. Metabolites were separated by paper chromatography and thin layer chromatography. Androstenedione, 5α-dihydrotestosterone, 5α-androstanedione, androsterone, and epiandrosterone were identified as metabolites. The metabolites were identified by reverse isotopic dilution and the preparation of derivatives (formation of acetates or reduction with NaBH4). Incubation of androstenedione-4-4C with human skin produced metabolites with similar chromatographic mobilities, and the formation of testosterone was established by reverse isotopic dilution. No radioactive etiocholanolone or etiocholanedione could be detected among the metabolites. © 1968, American Chemical Society. All rights reserved.