INVITRO RELEASE OF [5-METHIONINE]ENKEPHALIN AND [5-LEUCINE]-ENKEPHALIN FROM RAT GLOBUS PALLIDUS

被引:45
作者
BAYON, A [1 ]
ROSSIER, J [1 ]
MAUSS, A [1 ]
BLOOM, FE [1 ]
IVERSEN, LL [1 ]
LING, N [1 ]
GUILLEMIN, R [1 ]
机构
[1] SALK INST BIOL STUDIES,NEUROENDOCRINOL LABS,SAN DIEGO,CA 92112
关键词
D O I
10.1073/pnas.75.7.3503
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Endogenous [5-methionine]enkephalin (Metenkephalin) and [5-leucine]enkephalin (Leu-enkephalin) are released from perfused slices of rat globus pallidus by increased K+ in a Ca2+-dependent manner. Tissue perfused for 40 min contained only 26% of the Met-enkephalin and 44% of the Leu-enkephalin found in the freshly dissected tissue. After perfusion, the mean (±SEM) ratio (wt/wt) of Met-enkephalin to Leu-enkephalin was 3.4 ± 0.2 compared with 5.8 ± 0.2 in the fresh tissue. The degradation of trace amounts of synthetic [3H]enkephalins in the perfusing medium during stimulated release seems to reflect the accelerated degradation of enkephalin released from the tissue: 63% of the Met-enkephalin and 23% of the Leu-enkephalin were degraded in a medium containing bacitracin (30μg/ml). The mean ratio (wt/wt) of the Met-enkephalin to the Leu-enkephalin recovered after release by exposure of slices to 50 mM K+ was 2.7 ± 0.3. When perfusates were corrected for degradation, this ratio increased to about 5.5 which is higher than that found in the perfused tissue. The differences in release, tissue loss, and catabolism of the two enkephalins may be reflecting differences in the metabolic systems operating on the pentapeptides, but this interpretation will have to be validated by in vivo release experiments. In any event these observations strongly suggest that both enkephalins can be considered candidate neurotransmitters in the rat globus pallidus.
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页码:3503 / 3506
页数:4
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