DNA CHAIN-LENGTH MARKERS AND THE INFLUENCE OF BASE COMPOSITION ON ELECTROPHORETIC MOBILITY OF OLIGODEOXYRIBONUCLEOTIDES IN POLYACRYLAMIDE-GELS

被引：108

作者：

FRANK, R

KOSTER, H

机构：

[1] Institut für Organische Chemie und Biochemie, Universität Hamburg, D-2000 Hamburg 13

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 06期

关键词：

D O I：

10.1093/nar/6.6.2069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The specific influence of the four nucleobases on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels under denaturing and nondenaturing conditions has been investigated using homooligomers from the four deoxyribonucleotides as chain length standards. Homooligomers of same chain lengths exhibit remarkable differences in mobility. Specific retardation of any other oligonucleotide investigated was found to be mainly dependent on base composition but not on sequence. A simple procedure is presented for calculating mobilities relative to the standards on denaturing gels. This allows a reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures. The homooligomers also show the same differences in mobility on nondenaturing gels. The significance of this effect for strand separation is discussed. © 1979 Information Retrieval Limited.

引用

页码：2069 / 2087

页数：19

共 28 条

[1] SEPARATION OF OLIGONUCLEOTIDES, NUCLEOTIDES, AND NUCLEOSIDES ON COLUMNS OF POLYSTYRENE ANION-EXCHANGERS WITH SOLVENT SYSTEMS CONTAINING ETHANOL [J].