EFFECTIVE SPECIFIC ACTIVITY DILUTION IN LABELED DNA OF CULTURED MAMMALIAN CELLS

被引：7

作者：

BURKI, HJ

OKADA, S

机构：

来源：

BIOPHYSICAL JOURNAL | 1969年 / 9卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(69)86373-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Upon labeling the DNA of cultured mammalian cells with radio-active thymidine it was found that there was a large reduction in the specific activity of radioactive thymidine in DNA when this specific activity was compared to the specific activity of the exogenous supply. This reduction in specific activity may be determined once the effective dilution factor for thymidine in mammalian cells is known. The average effective dilution factor was found to be 2 × 10-4 M for L5178Y cells in log growth. © 1969, The Biophysical Society. All rights reserved.

引用

页码：122 / &

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