CRYSTALLIZATION OF A SOLUBLE FORM OF THE RAT T-CELL SURFACE GLYCOPROTEIN CD4 COMPLEXED WITH FAB FROM THE W3/25 MONOCLONAL-ANTIBODY

被引：29

作者：

DAVIS, SJ

BRADY, RL

BARCLAY, AN

HARLOS, K

DODSON, GG

WILLIAMS, AF

机构：

[1] UNIV YORK,YORK YO1 5DD,N YORKSHIRE,ENGLAND

[2] UNIV OXFORD,CTR MOLEC SCI,MOLEC BIOPHYS LAB,OXFORD OX1 3QU,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 213卷 / 01期

关键词：

D O I：

10.1016/S0022-2836(05)80116-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 Å and have the orthorhombic space group P21212 or P212121. The unit cell has dimensions a = 317 Å, b = 161 Å and c = 41·8 Å and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis. © 1990 Academic Press Limited.

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页码：7 / 10

页数：4

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