DISCRIMINATING INTRINSIC AND ANTIGEN-SELECTED MUTATIONAL HOTSPOTS IN IMMUNOGLOBULIN V-GENES

被引：216

作者：

BETZ, AG

NEUBERGER, MS

MILSTEIN, C

机构：

[1] Alexander G. Betz, Michael S. Neuberger and César Milstein are, the Medical Research Council Laboratory of Molecular Biology, Cambridge, CB2 2QH, Hills Road

来源：

IMMUNOLOGY TODAY | 1993年 / 14卷 / 08期

关键词：

D O I：

10.1016/0167-5699(93)90144-A

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Studies of the antibody hypermutation mechanism have revealed that it is not a random process but exhibits characteristic nucleotide substitution preferences. Here, Alexander Betz and colleagues show that these innate nucleotide substitution preferences can be used to examine databases of antigen-selected V gene sequences and thereby distinguish intrinsic from antigen-selected hotspots. This analysis reveals intrinsic mutational hotspots in both V(H) and V(L) genes, reflecting innate features of the hypermutation machinery which may give clues to the enzymatic mechanism.

引用

页码：405 / 411

页数：7

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