CONTROL OF REPLICATION OF PLASMID R1 - THE DUPLEX BETWEEN THE ANTISENSE RNA, COPA, AND ITS TARGET, COPT, IS PROCESSED SPECIFICALLY INVIVO AND INVITRO BY RNASE-III

被引:167
作者
BLOMBERG, P
WAGNER, EGH
NORDSTROM, K
机构
关键词
antisense RNA; nucleolytic processing; plasmid R1; replication control; RNase III;
D O I
10.1002/j.1460-2075.1990.tb07405.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The replication frequency of IncFII plasmids is regulated through the availability of a rate-limiting protein, RepA. The synthesis of this protein is controlled post-transcriptionally by a small antisense RNA, CopA, which binds to the leader region of the RepA mRNA (CopT). In this communication we report studies of the IncFII plasmid R1. We show that the duplex between CopA and CopT is cleaved specifically in vivo. The in vivo cleavage maps to the same position as that resulting from in vitro cleavage of a CopA/CopT duplex by purified RNase III. By introducing plasmids carrying translational repA-lacZ fusions into cells deficient in RNase III we show that the expression of repA is elevated when RNase III activity is severely decreased. Hence, cleavage by RNase III seems to be a key event in the copy number control system of plasmid R1.
引用
收藏
页码:2331 / 2340
页数:10
相关论文
共 41 条
[1]   PEDIGREES OF SOME MUTANT STRAINS OF ESCHERICHIA-COLI K-12 [J].
BACHMANN, BJ .
BACTERIOLOGICAL REVIEWS, 1972, 36 (04) :525-557
[2]   AUTO-REGULATION OF RNASE-III OPERON BY MESSENGER-RNA PROCESSING [J].
BARDWELL, JCA ;
REGNIER, P ;
CHEN, SM ;
NAKAMURA, Y ;
GRUNBERGMANAGO, M ;
COURT, DL .
EMBO JOURNAL, 1989, 8 (11) :3401-3407
[4]   CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].
CHANG, ACY ;
COHEN, SN .
JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156
[5]  
DEWILDE M, 1978, P NATL ACAD SCI USA, V75, P3673
[6]   PLASMID R1 DNA-REPLICATION DEPENDENT ON PROTEIN-SYNTHESIS IN CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI [J].
DIAZ, R ;
NORDSTROM, K ;
STAUDENBAUER, WL .
NATURE, 1981, 289 (5795) :326-328
[7]   TRANSCRIPTIONAL PAUSING IN A REGION IMPORTANT FOR PLASMID NR1 REPLICATION CONTROL [J].
DONG, XI ;
WOMBLE, DD ;
ROWND, RH .
JOURNAL OF BACTERIOLOGY, 1987, 169 (12) :5353-5363
[8]  
FITZWATER T, 1988, EMBO J, V7, P3279
[9]   SEQUENCE DIVERSITY AMONG RELATED GENES FOR RECOGNITION OF SPECIFIC TARGETS IN DNA-MOLECULES [J].
GOUGH, JA ;
MURRAY, NE .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (01) :1-19
[10]   THE ROLE OF ANTISENSE RNA IN GENE-REGULATION [J].
GREEN, PJ ;
PINES, O ;
INOUYE, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :569-597