RADIOACTIVE ASSAY FOR GUANIDOACETATE METHYLTRANSFERASE

被引：14

作者：

IM, YS

CANTONI, GL

CHIANG, PK

机构：

[1] Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90189-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A radioactive assay for guanidoacetate methyltransferase is described. It is based on a separation by an AG 50 (NH4+) column, on which [methyl-14C]Adenosylmethionine is adsorbed, in contrast to [methyl-14C]creatine which can be collected in the effluent fraction. Guanidoacetate methyltransferase is sensitive to inhibition by adenosylhomocysteine and 3-deazaadenosylhomocysteine. © 1979.

引用

页码：87 / 88

页数：2