TISSUE-SPECIFIC EXPRESSION OF A PLANT TURGOR-RESPONSIVE GENE WITH AMINO-ACID-SEQUENCE HOMOLOGY TO TRANSPORT-FACILITATING PROTEINS

被引：22

作者：

GUERRERO, FD ^{[1
]}

CROSSLAND, L ^{[1
]}

机构：

[1] CIBA GEIGY CORP, AGR BIOTECHNOL RES UNIT, RES TRIANGLE PK, NC 27709 USA

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 21卷 / 05期

关键词：

GENE PROMOTER; TRANSGENIC TOBACCO; TRANSPORT; TURGOR; WATER STRESS;

D O I：

10.1007/BF00027125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation of a turgor-responsive gene of pea, trg-31, whose transcription induced within 30 min after the loss of leaf turgor. Structure of the coding region and 1.4 kb of 5' untranslated region was determined by DNA sequencing. A 3 kb promoter fragment from trg-31 was fused to a beta-glucuronidase (GUS) reporter gene in pBI101, tobacco leaf disks and mature plants analyzed for turgor-responsive induction of GUS mRNA. Significant amino acid sequence homology exists between trg-31 and putative transport proteins of bovine, Phaseolus, soybean and Escherichia coli membranes.

引用

页码：929 / 935

页数：7

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