A STRAIN-SPECIFIC MODIFIER ON MOUSE CHROMOSOME-4 CONTROLS THE METHYLATION OF INDEPENDENT TRANSGENE LOCI

被引：190

作者：

ENGLER, P ^{[1
]}

HAASCH, D ^{[1
]}

PINKERT, CA ^{[1
]}

DOGLIO, L ^{[1
]}

GLYMOUR, M ^{[1
]}

BRINSTER, R ^{[1
]}

STORB, U ^{[1
]}

机构：

[1] UNIV PENN, SCH VET MED, PHILADELPHIA, PA 19104 USA

来源：

CELL | 1991年 / 65卷 / 06期

关键词：

D O I：

10.1016/0092-8674(91)90546-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transgene, pHRD, is highly methylated in 12 independent mouse lines when in a C57BL/6 strain background, but becomes progressively less methylated when bred into a DBA/2 background. Transgenes inherited from the mother are generally more methylated; however, this parental effect disappears following continued breeding into the nonmethylating strain. Mapping experiments using BXD recombinant inbred mice as well as other inbred strains indicate that a single strain-specific modifier (Ssm-1) linked to, but distinct from, Fv-1 is responsible for the strain effect. In addition to the methylated and unmethylated transgenic phenotypes, certain mice exhibit a partial methylation pattern that is a consequence of an unusual cellular mosaicism. The pHRD transgene, containing target sequences for the V(D)J recombinase, undergoes site-specific recombination only in lymphoid tissues. This V-J joining is restricted primarily to unmethylated transgene copies.

引用

页码：939 / 947

页数：9

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