SH3 DOMAIN-MEDIATED DIMERIZATION OF AN N-TERMINAL FRAGMENT OF THE PHOSPHATIDYLINOSITOL 3-KINASE P85 SUBUNIT

被引：9

作者：

CHEN, JK ^{[1
]}

SCHREIBER, SL ^{[1
]}

机构：

[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 14期

关键词：

D O I：

10.1016/S0960-894X(00)80375-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A recombinant fragment of the phosphatidylinositol 3-kinase p85 subunit containing the SH3 domain and an adjacent proline-rich region was analyzed by fluorescence spectroscopy and size exclusion chromatography. The recombinant protein dimerizes in solution with a dissociation constant of 0.9 +/- 0.3 muM.

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页码：1755 / 1760

页数：6

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