NMDA-RECEPTOR MEDIATED ELECTRICAL EPILEPTOGENESIS IN THE ORGANOTYPIC CULTURE OF RAT HIPPOCAMPUS

被引：6

作者：

SHIN, C

TAMAKI, Y

WILSON, JT

BUTLER, L

SAKAGUCHI, T

机构：

[1] DEPT VET AFFAIRS MED CTR, EPILEPSY RES LAB, DURHAM, NC 27705 USA

[2] DUKE UNIV, MED CTR, DEPT MED NEUROL, DURHAM, NC 27705 USA

来源：

BRAIN RESEARCH | 1992年 / 589卷 / 01期

关键词：

SEIZURE; N-METHYL-D-ASPARTATE; ORGANOTYPIC CULTURE; HIPPOCAMPUS; EPILEPTOGENESIS; KINDLING;

D O I：

10.1016/0006-8993(92)91171-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Extracellular field recordings were made in CA1 in the hippocampal explant cultures in oxygenated artificial cerebrospinal fluid. Schaffer collaterals were stimulated with 1-s trains of 60 Hz pulses every 10 min. Seizures were reliably elicited with progressive lengthening over 1-2 h. D-APV, an N-methyl-D-aspartate (NMDA) antagonist, stereoselectively blocked the development of seizures. Thus we have demonstrated that in vitro epileptogenesis occurs in hippocampal explant cultures through NMDA receptor mediated mechanisms.

引用

页码：129 / 134

页数：6

共 28 条

[1]

BRADY R J, 1989, Epilepsia, V30, P672

[2] MORPHOLOGICAL ORGANIZATION OF RAT HIPPOCAMPAL SLICE CULTURES [J].