THE FUNCTIONAL-CHARACTERIZATION OF INTERLEUKIN-10 RECEPTOR EXPRESSION ON HUMAN NATURAL-KILLER-CELLS

被引:166
作者
CARSON, WE
LINDEMANN, MJ
BAIOCCHI, R
LINETT, M
TAN, JC
CHOU, CC
NARULA, S
CALIGIURI, MA
机构
[1] ROSWELL PK CANC INST,DEPT MED,BUFFALO,NY 14263
[2] ROSWELL PK CANC INST,DEPT SURG,BUFFALO,NY 14263
[3] ROSWELL PK CANC INST,DEPT MOLEC MED,BUFFALO,NY 14263
[4] SCHERING PLOUGH CORP,RES INST,KENILWORTH,NJ
关键词
D O I
10.1182/blood.V85.12.3577.bloodjournal85123577
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Human natural killer (NK) cells are large granular lymphocytes that constitutively express functional forms of the interleukin-a receptor (IL-2R) and lyse tumor and virally infected cells without prior sensitization. NK cells with high density expression of CD56 (CD56(bright)) express the high affinity IL-2R and proliferate in response to low (picomolar) concentrations of IL-2. CD56(dim) NK cells express the intermediate affinity IL-2R and demonstrate enhanced cytotoxic activity without proliferation in response to high (nanomolar) concentrations of IL-2. In the present study, we characterized IL-10R expression on human NK cells and the functional consequences of IL-10 binding directly to highly purified subsets of CD56(bright) and CD56(dim) NK cells. Binding studies using I-125-IL-10 indicated that resting human NK cells constitutively express the IL-10 receptor protein at a surface density of approximately 90 receptor sites per cell, with a kd of similar to 1 nmol/L. Alone, IL-10 did not induce proliferation of CD56(bright) or CD56(dim) NK cell subsets. However, at low concentrations (0.5 to 5 ng/mL), IL-10 significantly augmented IL-2-induced proliferation of the CD56(bright) NK cell subset mediated via the high-affinity IL-2R. In the absence of IL-2, IL-10 was able to induce significant NK cytotoxic activity against NK-resistant tumor cell targets in both subsets of NK cells in a dose-dependent fashion. Furthermore, the combination of IL-10 and IL-2 had an additive effect on NK cytotoxic activity, whereas that of IL-10 and IL-12 did not. Production of interferon-gamma, tumor necrosis factor-alpha, and granulocyte-macrophage colony-stimulating factor by IL-2-activated NK cells was also significantly enhanced by IL-10. Neither resting nor activated human NK cells appear to produce human IL-10 protein. In summary, NK cells constitutively express the IL-10R protein in low density, and the functional consequences of IL-10 binding directly to human NK cell subsets appear to be stimulatory and dose-dependent. In contrast to its direct effects on human T cells and monocytes/macrophages. IL-10 potentiates cytokine production by human NK cells. (C) 1995 by The American Society of Hematology.
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页码:3577 / 3585
页数:9
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