CELL MICROFLUOROMETRY - A METHOD FOR RAPID FLUORESCENCE MEASUREMENT

被引:424
作者
VANDILLA, MA
TRUJILLO, TT
MULLANEY, PF
COULTER, JR
机构
[1] Biomedical Research Group, Los Alamos Scientific Laboratory, University of California, Los Alamos
关键词
D O I
10.1126/science.163.3872.1213
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A high-speed flow system for quantitative determination of fluoresence of cells containing fluorochrome has been developed. Feulgen-DNA distributions in populations of tissue culture cells and human leukocytes have been measured at a rate of 104 to 105 cells per minute and compare well with results of other independent methods.
引用
收藏
页码:1213 / &
相关论文
共 13 条
[1]   DEOXYRIBONUCLEIC ACID CONTENT OF MITOTIC NUCLEI OF 2 MOUSE TUMOURS [J].
ALPEN, EL ;
JOHNSTON, ME .
NATURE, 1966, 211 (5050) :715-&
[2]  
BARGER JD, 1961, AM J CLIN PATHOL, V36, P182
[3]   A DEVICE FOR COUNTING SMALL PARTICLES SUSPENDED IN A FLUID THROUGH A TUBE [J].
CROSLANDTAYLOR, PJ .
NATURE, 1953, 171 (4340) :37-38
[4]   ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME [J].
FULWYLER, MJ .
SCIENCE, 1965, 150 (3698) :910-&
[5]   SCHIFF-TYPE REAGENTS IN CYTOCHEMISTRY .1. THEORETICAL AND PRACTICAL CONSIDERATIONS [J].
KASTEN, FH .
HISTOCHEMIE, 1959, 1 (06) :466-509
[6]  
MULLANEY PF, 1968, 3 INT C HIST CYT NEW, P186
[7]   MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS [J].
ROTMAN, B ;
PAPERMASTER, BW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (01) :134-+
[8]  
RUCH F, 1966, INTRODUCTION QUANTIT
[9]   GENETICS OF SOMATIC MAMMALIAN CELLS .2. CHROMOSOMAL CONSTITUTION OF CELLS IN TISSUE CULTURE [J].
TJIO, JH ;
PUCK, TT .
JOURNAL OF EXPERIMENTAL MEDICINE, 1958, 108 (02) :259-&
[10]   PROPERTIES OF MITOTIC CELLS PREPARED BY MECHANICALLY SHAKING MONOLAYER CULTURES OF CHINESE HAMSTER CELLS [J].
TOBEY, RA ;
ANDERSON, EC ;
PETERSEN, DF .
JOURNAL OF CELLULAR PHYSIOLOGY, 1967, 70 (01) :63-&