PK(A) OF THE PROTONATED SCHIFF-BASE OF BOVINE RHODOPSIN - A STUDY WITH ARTIFICIAL PIGMENTS

被引：80

作者：

STEINBERG, G ^{[1
]}

OTTOLENGHI, M ^{[1
]}

SHEVES, M ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,DEPT PHYS CHEM,IL-91904 JERUSALEM,ISRAEL

来源：

BIOPHYSICAL JOURNAL | 1993年 / 64卷 / 05期

关键词：

D O I：

10.1016/S0006-3495(93)81518-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Artificial bovine rhodopsin pigments derived from synthetic retinal analogues carrying electron-withdrawing substituents (fluorine and chlorine) were prepared. The effects of the electron withdrawing substituents on the pKa values of the pigments and on the corresponding Schiff bases in solution were analyzed. The data suggest that the apparent pKa of the protonated Schiff base is above 16. However, the alternative possibility that the retinal Schiff base linkage in bovine rhodopsin is not accessible for titration from the aqueous bulk medium cannot be definitely ruled out. © 1993, The Biophysical Society. All rights reserved.

引用

页码：1499 / 1502

页数：4

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