IMMUNO-CYTOCHEMICAL DETECTION OF B-CELLS IN THE GUINEA-PIG PANCREAS USING GUINEA-PIG ANTISERUM TO PORCINE INSULIN

被引：18

作者：

BASKIN, DG

GORRAY, KC

FUJIMOTO, WY

机构：

[1] UNIV WASHINGTON, SCH MED, DEPT BIOL STRUCT, SEATTLE, WA 98195 USA

[2] VET ADM HOSP, MED CTR, DIV ENDOCRINE METAB, SEATTLE, WA 98108 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1981年 / 29卷 / 04期

关键词：

D O I：

10.1177/29.4.7019308

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study determined if antisera to an heterologous insulin could replace specific anti-guinea pig insulin serum for immunoperoxidase localization of guniea pig B cells. Antisera to porcine insulin was effective for this purpose, even though it was produced by guinea pigs. The immunostaining results obtained with anti-porcine insulin sera and anti-guinea pig insulin serum were identical, by both light microscopic and EM immunoperoxidase staining. The guinea pig antisera to porcine insulin apparently contained antibodies that reacted with guinea pig insulin.

引用

页码：567 / 571

页数：5

共 22 条

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