STREPTOCOCCUS-PNEUMONIAE PRODUCES AT LEAST 2 DISTINCT ENZYMES WITH NEURAMINIDASE ACTIVITY - CLONING AND EXPRESSION OF A 2ND NEURAMINIDASE GENE IN ESCHERICHIA-COLI

被引：26

作者：

CAMARA, M ^{[1
]}

MITCHELL, TJ ^{[1
]}

ANDREW, PW ^{[1
]}

BOULNOIS, GJ ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT MICROBIOL,POB 138,MED SCI BLDG,UNIV RD,LEICESTER LE1 9HN,ENGLAND

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 08期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/IAI.59.8.2856-2858.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A gene from Streptococcus pneumoniae was cloned in lambda EMBL301 and then expressed in Escherichia coli, which cleaved the fluorogenic neuraminidase substrate 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. The cloned gene therefore encodes an enzyme with neuraminidase activity. On the basis of restriction mapping and DNA hybridization studies, this gene could be distinguished from another pneumococcal neuraminidase gene cloned previously (A. M. Berry, J. C. Paton, E. M. Glare, D. Hansman, and D. E. A. Catcheside, Gene 71:299-305, 1988). Both neuraminidase genes were found in each of five isolates, covering at least three serotypes, of pneumococci tested.

引用

页码：2856 / 2858

页数：3

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