FUNCTIONAL NEUROANATOMICAL MAPPING IN INSECTS BY [H-3]-LABELED 2-DEOXY-D-GLUCOSE AT ELECTRON MICROSCOPICAL RESOLUTION

被引：11

作者：

BUCHNER, S ^{[1
]}

BUCHNER, E ^{[1
]}

机构：

[1] MAX PLANCK INST BIOL KYBERNETIK, SPEMANNSTR 38, D-7400 TUBINGEN, GERMANY

来源：

NEUROSCIENCE LETTERS | 1982年 / 28卷 / 03期

关键词：

D O I：

10.1016/0304-3940(82)90063-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

EM autoradiography of insect [Drosophila] nervous tissue prepared for activity staining by the deoxyglucose method provides information on metabolic activity of small identifiable nerve fibers. Grain counts over 2 sets of axon profiles selected for high and low radioactivity, respectively, indicate that the dynamic range of the present method comprises .apprx. 1 order of magnitude. Although a small fraction of the radioactive label is lost during flotation of the thin sections on a water surface, the distribution of the remaining label is not appreciably altered and reflects stimulus-induced nervous activity.

引用

页码：235 / 240

页数：6

共 11 条

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