MOUSE EGG INTEGRIN ALPHA-6-BETA-1 FUNCTIONS AS A SPERM RECEPTOR

被引：449

作者：

ALMEIDA, EAC

HUOVILA, APJ

SUTHERLAND, AE

STEPHENS, LE

CALARCO, PG

SHAW, LM

MERCURIO, AM

SONNENBERG, A

PRIMAKOFF, P

MYLES, DG

WHITE, JM

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT STOMATOL,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,DEPT ANAT,SAN FRANCISCO,CA 94143

[3] HARVARD UNIV,SCH MED,CANC BIOL LAB,BOSTON,MA 02115

[4] NETHERLANDS CANC INST,1066 CX AMSTERDAM,NETHERLANDS

[5] UNIV CONNECTICUT,DEPT PHYSIOL,FARMINGTON,CT 06030

来源：

CELL | 1995年 / 81卷 / 07期

关键词：

D O I：

10.1016/S0092-8674(05)80014-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding between sperm and egg plasma membranes is an essential step in fertilization. Whereas fertilin, a mammalian sperm surface protein, is involved in this crucial interaction, sperm receptors on the egg plasma membrane have not been identified. Because fertilin contains a predicted integrin ligand domain, we investigated the expression and function of integrin subunits in unfertilized mouse eggs. Polymerase chain reactions detected mRNAs for alpha 5, alpha 6, alpha v, beta 1, beta 3, and beta 5. Immunofluorescence revealed alpha 6 beta 1 and alpha v beta 3 on the plasma membrane. GoH3, a function-blocking anti-alpha 6 monoclonal antibody, abolished sperm binding, but a nonfunction-blocking anti-alpha 6 monoclonal antibody, a function-blocking anti-alpha v beta 3 polyclonal antibody, and an RGD peptide had no effect. Somatic cells bound sperm avidly, but only if they expressed alpha 6 beta 1. A peptide analog of the fertilin integrin ligand domain inhibited sperm binding to eggs and alpha 6 beta 1(+) cells and diminished GoH3 staining of eggs. Our results indicate a novel role for the integrin alpha 6 beta 1 as a cell-cell adhesion receptor that mediates sperm-egg binding.

引用

页码：1095 / 1104

页数：10

共 64 条

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