IDENTIFICATION AND CHARACTERIZATION OF SYN-ISOMERS AND ANTI-ISOMERS OF RETINALOXIMES

被引：60

作者：

GROENENDIJK, GWT

DEGRIP, WJ

DAEMEN, FJM

机构：

[1] Department of Biochemistry, University of Nijmegen

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80011-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydroxylamine derivatization of retinals affords two reaction products in a ratio of about 3:1. Preparative high-pressure liquid chromatography allows isolation of both products in a pure form. Mass spectroscopy and nuclear magnetic resonance spectroscopy allow identification. The main products possess the syn, the minor products the anti configuration. © 1979 Academic Press, Inc.

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页码：304 / 310

页数：7

相关论文

共 14 条

[1] RHODOPSIN CYCLE [J].

DAEMEN, FJM ;

ROTMANS, JP ;

BONTING, SL .

EXPERIMENTAL EYE RESEARCH, 1974, 18 (01) :97-103

[2]

FUTTERMAN A, 1974, BIOCHIM BIOPHYS ACTA, V337, P390, DOI 10.1016/0005-2760(74)90114-3

[3]

GRIP WJD, 1972, VISION RES, V12, P1697

[4]

HANTZSCH, 1891, BER DEUT CHEM GES, V24, P3480

[5]

HUBBARD R., 1971, Methods in Enzymology, V18C, P615, DOI 10.1016/S0076-6879(71)18045-7

[6] STRUCTURAL STUDIES BY NUCLEAR MAGNETIC RESONANCE .3. SYN-ANTI ASSIGNMENTS FROM SOLVENT EFFECTS [J].

KARABATSOS, GJ ;

TALLER, RA ;

VANE, FM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (15) :2327-&

[7] STRUCTURAL STUDIES BY NUCLEAR MAGNETIC RESONANCE .11. CONFORMATIONS AND CONFIGURATIONS OF OXIME O-METHYL ETHERS [J].

KARABATSOS, GJ ;

HSI, N .

TETRAHEDRON, 1967, 23 (03) :1079-+

[8]

LEENHEER APD, 1975, ANAL BIOCHEM, V63, P169

[9] BIOCHEMICAL ASPECTS OF VISUAL PROCESS .27. STEREOSPECIFICITY OF OCULAR RETINOL DEHYDROGENASES AND VISUAL CYCLE [J].

LION, F ;

ROTMANS, JP ;

DAEMEN, FJM ;

BONTING, SL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 384 (02) :283-292

[10]

METZGER H, 1968, METHODEN ORGANISCHEN, V10, P282