PROPERTIES OF FORMALDEHYDE-TREATED NUCLEOHISTONE

被引：187

作者：

BRUTLAG, D

SCHLEHUB.C

BONNER, J

机构：

[1] Division of Biology, California Institute of Technology, Pasadena, California

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 08期

关键词：

D O I：

10.1021/bi00836a013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When native nucleohistone is treated with formaldehyde, the resulting soluble complex does not dissociate in solutions of high ionic strength and may be banded in a CsCl gradient. The buoyant density of the complex, 1.411 g/cc, is that calculated from the ratio of deoxyribonucleic acid to histone in nucleohistone. Formaldehyde treatment of nucleohistone cross-links histone molecules to deoxyribonucleic acid. Thus the histone of the formaldehyde-treated complex is no longer soluble in acid. Treatment with pronase removes 96% of the protein from deoxyribonucleic acid, leaving 4% firmly bound, an amount which is in approximate stoichiometric proportion to the formaldehyde cross-links between deoxyribonucleic acid and protein. © 1969, American Chemical Society. All rights reserved.

引用

页码：3214 / &

共 10 条

[1]

Bonner J, 1968, METHODS ENZYMOL, V12, P3

[2]

BRUNNER R, 1965, BIOCHIM BIOPHYS ACTA, V108, P5

[3] NET HYDRATION OF T-4 BACTERIOPHAGE DEOXYRIBONUCLEIC ACID AND EFFECT OF HYDRATION ON BUOYANT BEHAVIOR IN A DENSITY GRADIENT AT EQUILIBRIUM IN ULTRACENTRIFUGE [J].