CLONING OF THE ACTIVE THYMIDINE KINASE GENE OF HERPES-SIMPLEX VIRUS TYPE-1 IN ESCHERICHIA-COLI K-12

被引：286

作者：

COLBEREGARAPIN, F

CHOUSTERMAN, S

HORODNICEANU, F

KOURILSKY, P

GARAPIN, AC

机构：

[1] INST PASTEUR, UNITE GEN GENET, BIOL MOLEC GENE GRP, F-75724 PARIS, FRANCE

[2] INST RECH SCI CANC, F-94800 VILLEJUIF, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 08期

关键词：

D O I：

10.1073/pnas.76.8.3755

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinant plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.

引用

页码：3755 / 3759

页数：5

共 24 条

[1] TRANSFER OF GENE FOR THYMIDINE KINASE TO THYMIDINE KINASE-DEFICIENT HUMAN CELLS BY PURIFIED HERPES-SIMPLEX VIRAL-DNA [J].