MEASURING CIRCULATING CYTOKINES

被引：92

作者：

CANNON, JG ^{[1
]}

NERAD, JL ^{[1
]}

POUTSIAKA, DD ^{[1
]}

DINARELLO, CA ^{[1
]}

机构：

[1] USDA,HUMAN NUTR RES CTR AGING,BOSTON,MA 02111

来源：

JOURNAL OF APPLIED PHYSIOLOGY | 1993年 / 75卷 / 04期

关键词：

INTERLEUKIN-1; TUMOR NECROSIS FACTOR; BIOASSAY; IMMUNOASSAY; PLASMA; SERUM;

D O I：

10.1152/jappl.1993.75.4.1897

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Cytokines, i.e., regulatory proteins derived primarily (but not exclusively) from cells of the immune system, are receiving increasing attention for their influences on physiological processes. This paper outlines several of the unique characteristics of cytokines and discusses the pitfalls encountered when measuring them in biological fluids. At present, each available assay has a combination of advantages and drawbacks; therefore, investigators must be aware of the trade-offs and choose the assay that best addresses their needs. The factors that affect cytokine measurement also influence cytokine activity in vivo; thus they are important from a physiological as well as methodological standpoint. Moreover, the absolute concentration of a single cytokine is probably less important than the balance between that cytokine and its natural antagonists.

引用

页码：1897 / 1902

页数：6

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