PROTEIN TYROSINE PHOSPHATASE-ACTIVITY OF AN ESSENTIAL VIRULENCE DETERMINANT IN YERSINIA

被引：474

作者：

GUAN, KL

DIXON, JE

机构：

[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907

[2] PURDUE UNIV,WALTHER CANC INST,W LAFAYETTE,IN 47907

来源：

SCIENCE | 1990年 / 249卷 / 4968期

关键词：

D O I：

10.1126/science.2166336

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Yersinia is the genus of bacteria that is the causative agent in plague or the black death, and on several occasions this organism has killed a significant portion of the world's population. An essential virulence determinant of Yersinia was shown to be a protein tyrosine phosphatase. The recombinant 50-kilodalton Yersinia phosphatase had a specificity for removal of phosphate from Tyr-containing as opposed to Ser/Thr-containing phosphopeptides and proteins. Site-directed mutagenesis was used to show that the Yersinia phosphatase possesses an essential Cys residue required for catalysis. Amino acids surrounding an essential Cys residue are highly conserved, as are other amino acids in the Yersinia and mammalian protein tyrosine phosphatases, suggesting that they use a common catalytic mechanism.

引用

收藏

页码：553 / 556

页数：4

相关论文

共 24 条

[1] THE MOLECULAR-GENETICS OF CANCER [J].

BISHOP, JM .

SCIENCE, 1987, 235 (4786) :305-311

[2] THE PLASMID-ENCODED YOP2B PROTEIN OF YERSINIA-PSEUDOTUBERCULOSIS IS A VIRULENCE DETERMINANT REGULATED BY CALCIUM AND TEMPERATURE AT THE LEVEL OF TRANSCRIPTION [J].

BOLIN, I ;

WOLFWATZ, H .

MOLECULAR MICROBIOLOGY, 1988, 2 (02) :237-245

[3]

BUTLER T, 1985, TXB MED, P1600

[4] HUMAN-PLACENTA PROTEIN-TYROSINE-PHOSPHATASE - AMINO-ACID SEQUENCE AND RELATIONSHIP TO A FAMILY OF RECEPTOR-LIKE PROTEINS [J].

CHARBONNEAU, H ;

TONKS, NK ;

KUMAR, S ;

DILTZ, CD ;

HARRYLOCK, M ;

COOL, DE ;

KREBS, EG ;

FISCHER, EH ;

WALSH, KA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5252-5256

[5] THE LEUKOCYTE COMMON ANTIGEN (CD45) - A PUTATIVE RECEPTOR-LINKED PROTEIN TYROSINE PHOSPHATASE [J].

CHARBONNEAU, H ;

TONKS, NK ;

WALSH, KA ;

FISCHER, EH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7182-7186

[6] CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY [J].

COOL, DE ;

TONKS, NK ;

CHARBONNEAU, H ;

WALSH, KA ;

FISCHER, EH ;

KREBS, EG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5257-5261

[7] THE YERSINIA-YOP REGULON [J].

CORNELIS, GR ;

BIOT, T ;

DEROUVROIT, CL ;

MICHIELS, T ;

MULDER, B ;

SLUITERS, C ;

SORY, MP ;

VANBOUCHAUTE, M ;

VANOOTEGHEM, JC .

MOLECULAR MICROBIOLOGY, 1989, 3 (10) :1455-1459

[8] FISSION YEAST P13 BLOCKS MITOTIC ACTIVATION AND TYROSINE DEPHOSPHORYLATION OF THE XENOPUS CDC2 PROTEIN-KINASE [J].

DUNPHY, WG ;

NEWPORT, JW .

CELL, 1989, 58 (01) :181-191

[9] TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS [J].

GOULD, KL ;

NURSE, P .

NATURE, 1989, 342 (6245) :39-45

[10] CLONING AND EXPRESSION OF A PROTEIN-TYROSINE-PHOSPHATASE [J].

GUAN, KL ;

HAUN, RS ;

WATSON, SJ ;

GEAHLEN, RL ;

DIXON, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1501-1505