EXPRESSION IN ESCHERICHIA-COLI OF CHEMICALLY SYNTHESIZED GENES FOR HUMAN INSULIN

被引：671

作者：

GOEDDEL, DV ^{[1
]}

KLEID, DG ^{[1
]}

BOLIVAR, F ^{[1
]}

HEYNEKER, HL ^{[1
]}

YANSURA, DG ^{[1
]}

CREA, R ^{[1
]}

HIROSE, T ^{[1
]}

KRASZEWSKI, A ^{[1
]}

ITAKURA, K ^{[1
]}

RIGGS, AD ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR, DIV BIOL, DUARTE, CA 91010 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 01期

关键词：

D O I：

10.1073/pnas.76.1.106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an E. coli β-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from β-galactosidase, detected by radioimmunoassay, and purified. Complete purification of the A chain and partial purification of the B chain were achieved. These products were mixed, reduced, and reoxidized. The presence of insulin was detected by radioimmunoassay.

引用

页码：106 / 110

页数：5

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