AMPLIFICATION OF P450C21 EXPRESSION IN CULTURED-MAMMALIAN-CELLS

被引：6

作者：

RICKETTS, MH ^{[1
]}

CHIAO, E ^{[1
]}

HU, MC ^{[1
]}

CHUNG, BC ^{[1
]}

机构：

[1] ACAD SINICA, INST MOL BIOL, TAIPEI 115, TAIWAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 186卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80825-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. With the exception of MMTV-LTR, all drove the expression of similar levels of functional 21-hydroxylase. In addition, the Rat-1 cell line was tested and shown to be suitable for the stable expression of functional P450c21. We have established cell lines derived from Rat-1 either normal or mutant P450c21 stably expressed together with amplifiable markers. The expression of P450c21 was further increased by selection in methotrexate. © 1992 Academic Press, Inc.

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页码：426 / 431

页数：6

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