REQUIREMENT FOR BIOTIN IN MOUSE FIBROBLAST L-CELLS CULTURED ON SERUMLESS MEDIUM

被引：15

作者：

HAGGERTY, DF

SATO, GH

机构：

[1] Department of Biological Chemistry UCLA School of Medicine Los Angeles

[2] Graduate Department, Biochemistry Brandeis University Waltham, MA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 34卷 / 06期

关键词：

D O I：

10.1016/0006-291X(69)90252-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse L-cells, Clone 929, were adapted to growth on a chemically defined medium, Waymouth's Medium MAP 954 1, in the absence of serum. When biotin was excluded in the preparation of this medium, the resulting medium proved to be incapable of sustaining continuous cell proliferation: A biotin requirement was therefore demonstrable in these cells within a three week period. © 1969.

引用

页码：812 / &

共 14 条

[1] NUTRITIONAL REQUIREMENTS OF MOUSE ADRENAL CORTEX TUMOR CELLS IN CULTURE [J].