HUMAN PLACENTAL CHOLESTEROL SIDE-CHAIN CLEAVAGE - ENZYMATIC-SYNTHESIS OF (22R)-20-ALPHA,22-DIHYDROXYCHOLESTEROL

被引：22

作者：

TUCKEY, RC

CAMERON, KJ

机构：

[1] Department of Biochemistry, University of Western Australia, Nedlands

来源：

STEROIDS | 1993年 / 58卷 / 05期

关键词：

STEROIDS; CYTOCHROME-P450(SCC); PLACENTAL MITOCHONDRIA; (22R)-20-ALPHA; 22-DIHYDROXYCHOLESTEROL; (22R)-22-HYDROXYCHOLESTEROL; PREGNENOLONE;

D O I：

10.1016/0039-128X(93)90024-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(22R)-20alpha,22-Dihydroxycholesterol is the second intermediate in the conversion of cholesterol to pregnenolone by cytochrome P450scc in steroidogenic tissues. We report a rapid method for the enzymatic synthesis of (22R)-20alpha,22-dihydroxycholesterol from (22R)-22-hydroxycholesterol using mitochondria from the human placenta.

引用

页码：230 / 233

页数：4

共 14 条

[1] SIDE-CHAIN CLEAVAGE OF HYDROXYLATED STEROLS BY BOVINE ADRENAL-CORTEX MITOCHONDRIA - OBSERVATION OF 25-HYDROXYLASE ACTIVITY DURING INCUBATION AT PH 7.80 [J].