GENERATION AND CHARACTERIZATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MUTANTS

被引：87

作者：

ADACHI, A ^{[1
]}

ONO, N ^{[1
]}

SAKAI, H ^{[1
]}

OGAWA, K ^{[1
]}

SHIBATA, R ^{[1
]}

KIYOMASU, T ^{[1
]}

MASUIKE, H ^{[1
]}

UEDA, S ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,OSAKA,JAPAN

来源：

ARCHIVES OF VIROLOGY | 1991年 / 117卷 / 1-2期

关键词：

D O I：

10.1007/BF01310491

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17,p24, and p15 regions), pol(protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.

引用

页码：45 / 58

页数：14

共 40 条

[1] PRODUCTIVE, PERSISTENT INFECTION OF HUMAN COLORECTAL CELL-LINES WITH HUMAN-IMMUNODEFICIENCY-VIRUS [J].