TIP-LINK INTEGRITY AND MECHANICAL TRANSDUCTION IN VERTEBRATE HAIR-CELLS

被引：399

作者：

ASSAD, JA

SHEPHERD, GMG

COREY, DP

机构：

[1] HARVARD UNIV,SCH MED,DEPT NEUROBIOL,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,PROGRAM NEUROSCI,BOSTON,MA 02115

[3] MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114

[4] HOWARD HUGHES MED INST,NEUROSCI GRP,COCONUT GROVE,FL 33133

来源：

NEURON | 1991年 / 7卷 / 06期

关键词：

D O I：

10.1016/0896-6273(91)90343-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

An attractive hypothesis for hair-cell transduction is that fine, filamentous "tip links" pull directly on mechanically sensitive ion channels located dt the tips of the stereocilia. We tested the involvement of tip links in the transduction process by treating bundles with a BAPTA-buffered, low-Ca2+ saline (10(-9) M). BAPTA abolished the transduction current in a few hundred milliseconds. BAPTA treatment for a few seconds eliminated the tip links observed by either scanning or transmission electron microscopy. BAPTA also eliminated the voltage-dependent movement and caused a positive bundle displacement of 133 nm, in quantitative agreement with a model for regulation of tension. We conclude that tip links convey tension to the transduction channels of hair cells.

引用

页码：985 / 994

页数：10

共 32 条

[1] VOLTAGE DEPENDENCE OF ADAPTATION AND ACTIVE BUNDLE MOVEMENT IN BULLFROG SACCULAR HAIR-CELLS [J].