REDUCTION OF THE CALCIUM REQUIREMENT OF NORMAL HUMAN EPITHELIAL-CELLS BY EGF

被引：52

作者：

LECHNER, JF

KAIGHN, ME

机构：

[1] Pasadena Foundation for Medical Research, Pasadena

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 121卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0014-4827(79)90027-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The influence of epidermal growth factor (EGF) on the Ca2+ requirement of normal (NP-2s) and neoplastic human epithelial (PC-3) cells was studied using a clonal growth assay. The interaction of Ca2+ and EGF was investigated by kinetic analysis of dose-response experiments in which the Ca2+ or EGF concentration necessary for half-maximal growth, Km were determined. The normal epithelial cells required 80 times more calcium than did the cancer cells. In the presence of EGF, the Ca2+ requirement of both cell types was virtually identical. EGF did not affect the growth rate of the cancer line. The interaction between Ca2+and EGF was found to be unidirectional since EGF reduced the KmCa2+ 120-fold, whereas Ca2+ had no effect on the EGF dose-response. © 1979.

引用

页码：432 / 435

页数：4

共 14 条

[1] ROLES OF CALCIUM, SERUM, PLASMA, AND FOLIC-ACID IN CONTROL OF PROLIFERATION OF NORMAL AND ROUS-SARCOMA VIRUS-INFECTED CHICKEN FIBROBLASTS [J].