REDUCTION OF THE CALCIUM REQUIREMENT OF NORMAL HUMAN EPITHELIAL-CELLS BY EGF

被引:52
作者
LECHNER, JF
KAIGHN, ME
机构
[1] Pasadena Foundation for Medical Research, Pasadena
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0014-4827(79)90027-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The influence of epidermal growth factor (EGF) on the Ca2+ requirement of normal (NP-2s) and neoplastic human epithelial (PC-3) cells was studied using a clonal growth assay. The interaction of Ca2+ and EGF was investigated by kinetic analysis of dose-response experiments in which the Ca2+ or EGF concentration necessary for half-maximal growth, Km were determined. The normal epithelial cells required 80 times more calcium than did the cancer cells. In the presence of EGF, the Ca2+ requirement of both cell types was virtually identical. EGF did not affect the growth rate of the cancer line. The interaction between Ca2+and EGF was found to be unidirectional since EGF reduced the KmCa2+ 120-fold, whereas Ca2+ had no effect on the EGF dose-response. © 1979.
引用
收藏
页码:432 / 435
页数:4
相关论文
共 14 条
[1]   ROLES OF CALCIUM, SERUM, PLASMA, AND FOLIC-ACID IN CONTROL OF PROLIFERATION OF NORMAL AND ROUS-SARCOMA VIRUS-INFECTED CHICKEN FIBROBLASTS [J].
BALK, SD ;
WHITFIEL.JF ;
YOUDALE, T ;
BRAUN, AC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (03) :675-679
[2]   CONTROL OF HUMAN WI-38 CELL-PROLIFERATION BY EXTRACELLULAR CALCIUM AND ITS ELIMINATION BY SV40 VIRUS-INDUCED PROLIFERATIVE TRANSFORMATION [J].
BOYNTON, AL ;
WHITFIELD, JF ;
ISAACS, RJ ;
TREMBLAY, R .
JOURNAL OF CELLULAR PHYSIOLOGY, 1977, 92 (02) :241-247
[3]   INDUCTION OF GROWTH IN RESTING FIBROBLASTIC CELL-CULTURES BY CA++ [J].
DULBECCO, R ;
ELKINGTON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (04) :1584-1588
[4]   MECHANISM OF REGULATION OF FIBROBLASTIC CELL REPLICATION .4. ANALYSIS OF SERUM DEPENDENCE OF CELL REPLICATION BASED ON MICHAELIS-MENTEN KINETICS [J].
ELLEM, KAO ;
GIERTHY, JF .
JOURNAL OF CELLULAR PHYSIOLOGY, 1977, 92 (03) :381-400
[5]   DENSITY-DEPENDENT REGULATION OF GROWTH OF BSC-1 CELLS IN CELL-CULTURE - CONTROL OF GROWTH BY SERUM FACTORS [J].
HOLLEY, RW ;
ARMOUR, R ;
BALDWIN, JH ;
BROWN, KD ;
YEH, YC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :5046-5050
[6]  
KAIGHN ME, INVEST UROL
[7]   REPLICATIVE EPITHELIAL-CELL CULTURES FROM NORMAL HUMAN PROSTATE-GLAND [J].
LECHNER, JF ;
NARAYAN, KS ;
OHNUKI, Y ;
BABCOCK, MS ;
JONES, LW ;
KAIGHN, ME .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1978, 60 (04) :797-801
[8]  
LECHNER JF, 1978, J CELL BIOL, V79, pA66
[9]  
LECHNER JF, J CELL PHYSL
[10]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265