TESTICULAR LEYDIG-CELLS INVITRO SECRETE ONLY INHIBIN ALPHA-SUBUNITS, WHEREAS LEYDIG-CELL TUMORS CAN SECRETE BIOACTIVE INHIBIN

被引：42

作者：

DEWINTER, JP ^{[1
]}

TIMMERMAN, MA ^{[1
]}

VANDERSTICHELE, HMJ ^{[1
]}

KLAIJ, IA ^{[1
]}

GROOTENHUIS, AJ ^{[1
]}

ROMMERTS, FFG ^{[1
]}

DEJONG, FH ^{[1
]}

机构：

[1] INNOGENET,GHENT,BELGIUM

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 83卷 / 2-3期

关键词：

LEYDIG CELL; LEYDIG CELL TUMOR; INHIBIN; INHIBIN ALPHA-SUBUNIT; ACTIVIN; SERTOLI CELL;

D O I：

10.1016/0303-7207(92)90151-U

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The secretion of inhibin and inhibin-related proteins by testicular Leydig cells was studied by estimation of inhibin immunoreactivity and bioactivity in spent media of preparations of immature and mature rat Leydig cells and of tumor Leydig cells. Immature and mature rat Leydig cells expressed inhibin alpha-subunit mRNA and secreted immunoreactive inhibin. The immunoreactive material did not contain inhibin bioactivity as measured by an in vitro rat pituitary bioassay system. Results of pulse labeling with [S-35]methionine followed by immunoprecipitation indicated that the inhibin-related proteins secreted by the immature Leydig cell preparations are 26 kDa and 44 kDa molecules. Mature rat Leydig cells only secreted the 44 kDa inhibin-related protein. Tumor Leydig cells (rat H540 and mouse MA10) secreted immunoreactive and bioactive inhibin, which could be immunoneutralized by an antibody against inhibin. In the culture medium of some H540 tumor Leydig cells 26 kDa and 42 kDa inhibin-related proteins and 30 kDa inhibin were detected. In culture medium of other H540 tumor Leydig cells, not secreting bioactive inhibin, only 26 kDa and 42 kDa inhibin-related proteins were found. No activin bioactivity was detected in culture media of immature rat Leydig cells, H540 and MA10 tumor Leydig cells. It is concluded that normal Leydig cells secrete inhibin a-subunits, while Leydig cell tumors can also secrete bioactive inhibin. Neither normal Leydig cells nor Leydig cell tumors produce activin.

引用

页码：105 / 115

页数：11

共 45 条

[1] CHARACTERIZATION OF SEVERAL CLONAL LINES OF CULTURED LEYDIG TUMOR-CELLS - GONADOTROPIN RECEPTORS AND STEROIDOGENIC RESPONSES [J].