ISOLATION OF CHINESE HAMSTER OVARY CELLS WITH REDUCED POLY(ADP-RIBOSE) POLYMERASE-ACTIVITY

被引：17

作者：

MACLAREN, RA ^{[1
]}

WITMER, MV ^{[1
]}

RICHARDSON, E ^{[1
]}

STAMATO, TD ^{[1
]}

机构：

[1] WISTAR INST,PHILADELPHIA,PA 19104

来源：

MUTATION RESEARCH | 1990年 / 231卷 / 02期

关键词：

CHO cells; isolation; DNA repair; Poly(ADP-ribose) polymerase;

D O I：

10.1016/0027-5107(90)90032-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The biological function of poly(ADP-ribose) polymerase in DNA repair, cell-cycle regulation and cellular differentiation has yet to be defined. Isolation of cells which are deficient in poly(ADP-ribose) synthesis would greatly facilitate the determination of the biological role of this enzyme. A method is described for isolating Chinese hamster ovary (CHO) cells deficient in the poly(ADP-ribose) polymerase activity by direct screening of colonies for enzyme activity. Colonies with decreased production of poly(ADP-ribose) are recovered from nylon replicas for further analysis. Using this method we have isolated a series of CHO cells which have 50% or less poly(ADP-ribose) polymerase activity. These mutants have normal generation times and are 20% more sensitive to the effects of DNA (m)ethylating agents than the parental cell. However, these mutants display normal sensitivity to γ-rays. © 1990.

引用

页码：265 / 274

页数：10

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