MICROMETHOD FOR DETERMINING ADENOSINE-DEAMINASE AND PURINE NUCLEOSIDE PHOSPHORYLASE ACTIVITY IN CELLS FROM HUMAN PERIPHERAL-BLOOD

被引：34

作者：

VANDERWEYDEN, MB ^{[1
]}

BAILEY, L ^{[1
]}

机构：

[1] ALFRED HOSP,DEPT MED,PRAHRAN 3181,VICTORIA,AUSTRALIA

来源：

CLINICA CHIMICA ACTA | 1978年 / 82卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0009-8981(78)90041-4

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A radiochromatographic method is described for measuring adenosine deaminase and purine nucleoside phosphorylase activity in cells from human peripheral blood. The respective substrates, [8-14C]adenosine or [8-14Clinosine, are converted either to inosine and hypoxanthine or hypoxanthine, respectively. A single simple and rapid chromatographic procedure is used to isolate the products of both reactions. The mean normal activity (nmol h-1 mg-1) of ADA for erythrocytes is 63 ± 24 (± 1 S.D.) for leukocytes, 750 ± 280 and for lymphocytes, 2105 ± 1170. Corresponding activities for purine nucleoside phosphorylase are 1850 ± 490, 3665 ± 1170 and 5890 ± 2030. With the described methods a further patient with severe combined immunodeficiency and adenosine deaminase deficiency has been identified. © 1978.

引用

页码：179 / 184

页数：6

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