MOLECULAR EVIDENCE OF FETAL-DERIVED CHROMOSOME-21 MARKERS (STRS) IN TRANSCERVICAL SAMPLES

被引：25

作者：

ADINOLFI, M ^{[1
]}

SHERLOCK, J ^{[1
]}

SOOTHILL, P ^{[1
]}

RODECK, C ^{[1
]}

机构：

[1] UCL, SCH MED, DEPT OBSTET & GYNAECOL, LONDON WC1E, ENGLAND

来源：

PRENATAL DIAGNOSIS | 1995年 / 15卷 / 01期

关键词：

TRANSCERVICAL CELLS; FISH; SMALL TANDEM REPEATS; ENDOCERVICAL CANAL;

D O I：

10.1002/pd.1970150108

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Transcervical cells (TCCs), collected by flushing or aspiration at 8-13 weeks of gestation, were analysed for the presence of fetal-derived DNA sequences. DNA extracted from maternal peripheral blood, TCC samples, and placental tissue was amplified by the polymerase chain reaction (PCR) to detect small tandem repeat (STR) markers specific to chromosome 21. STR products of fetal origin could be clearly observed in four TCC samples. TCC samples collected by flushing or aspiration were also analysed by fluorescent in situ hybridization (FISH) using X and Y probes simultaneously: 46,XY cells could be detected in all TCC samples obtained from mothers with male fetuses.

引用

页码：35 / 39

页数：5

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