GLYCOPROTEIN DEGRADATION - GLYCOSIDASES IN FIBROBLASTS TRANSFORMED BY ONCOGENIC VIRUSES

Fibroblasts transformed by oncogenic viruses were found to have greater enzymic activities of 7 glycosidases on a cell or protein basis than two non-transformed cell lines. Two glycosidases studied (α-d-fucosidase and β-d-xylosidase) were not found in any of the fibroblast cells or HeLa cells. The glycosidase with the greatest activity in all the cells studied was N-acetyl-β-d-glucosaminidase. The most pronounced differences were found with α-d-glucosidase in which increases in activity in the transformed cells ranged from 3.1 to 4.5 fold over the non-transformed cell lines. © 1969.