TN10 MEDIATED INTEGRATION OF THE PLASMID R100.1 INTO THE BACTERIAL CHROMOSOME - INVERSE TRANSPOSITION

被引：34

作者：

CHANDLER, M

ROULET, E

SILVER, L

BOYDELATOUR, E

CARO, L

机构：

[1] Département de Biologie Moléculaire, Université de Genève, Geneve 4, CH-1211, 30, quai Ernest-Ansermet

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 173卷 / 01期

关键词：

D O I：

10.1007/BF00267687

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Upon integration into the bacterial chromosome the drug resistance plasmid R100.1 often loses its tetracycline resistance character. We have analyzed an Hfr strain formed by such an integration and an R-prime plasmid derived from it. We find that integration took place within the Tn10 transposon, that the two IS10 sequences were retained, but that at least 80% of the transposon segment located between them, and carrying the tetracycline resistance genes, had been lost. We suggest that integration of R100.1 was mediated by an inverse transposition using the IS10 sequences. © 1979 Springer-Verlag.

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页码：23 / 30

页数：8

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