SUSCEPTIBILITY OF HUMAN-MOUSE T-CELL HYBRIDS TO HIV-PRODUCTIVE INFECTION

被引：12

作者：

RAMARLI, D

CAMBIAGGI, C

MORGHEN, CD

TRIPPUTI, P

ORTOLANI, R

BOLZANELLI, M

TRIDENTE, G

ACCOLLA, RS

机构：

[1] UNIV VERONA,IST IMMUNOL & MALATTIE INFETT,I-37100 VERONA,ITALY

[2] UNIV MILAN,DIPARTIMENTO FARMACOL,I-20100 MILAN,ITALY

[3] UNIV MILAN,IST SCI BIOMED,OSPED S GERARDO,I-20052 MONZA,ITALY

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 12期

关键词：

D O I：

10.1089/aid.1993.9.1269

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-III(B) infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA-IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4+ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4+ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.

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页码：1269 / 1275

页数：7

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