LYSOPHOSPHATIDIC ACID-INDUCED ACTIVATION OF PROTEIN SER/THR KINASES IN CULTURED RAT 3Y1 FIBROBLASTS - POSSIBLE INVOLVEMENT IN RHO-P21-MEDIATED SIGNALING

被引：21

作者：

KUMAGAI, N ^{[1
]}

MORII, N ^{[1
]}

ISHIZAKI, T ^{[1
]}

WATANABE, N ^{[1
]}

FUJISAWA, K ^{[1
]}

SAITO, Y ^{[1
]}

NARUMIYA, S ^{[1
]}

机构：

[1] KYOTO UNIV, FAC MED, DEPT PHARMACOL, SAKYO KU, KYOTO 606, JAPAN

来源：

FEBS LETTERS | 1995年 / 366卷 / 01期

关键词：

RHO P21; LYSOPHOSPHATIDIC ACID; PROTEIN SER/THR KINASE; BOTULINUM C3 EXOENZYME; PERTUSSIS TOXIN; RAT; 3Y1; FIBROBLAST;

D O I：

10.1016/0014-5793(95)00478-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Renaturation kinase assay was used to detect protein kinases activated by lysophosphatidic acid (LPA) in cultured rat 3Y1 fibroblasts. LPA activated several Ser/Thr protein kinases with apparent molecular weights of 145K, 85K, 64-65K (a doublet), and 60K (each named p145, p85, p64165 and p60, respectively) in addition to p43 mitogen activated protein (MAP)kinase. Experiments using pertussis toxin and botulinum C3 exoenzyme showed that p145, p85, and p64165 kinases were activated by a pertussis toxin-insensitive rho p21-dependent pathway and that the activation of MAP-kinase was mediated by both the pertussis toxin-sensitive rho p21-independent and the pertussis toxin-insensitive rho p21-dependent pathways.

引用

页码：11 / 16

页数：6

共 33 条

[1]

Moolenaar, Trends Cell Biol., 4, pp. 213-219, (1994)

[2]

Ridley, Hall, Cell, 70, pp. 389-399, (1992)

[3]

Narumiya, Morii, Cell. Signalling, 5, pp. 9-19, (1993)

[4]

Morii, Teruuchi, Tominaga, Kumagai, Kozaki, Ushikubi, Narumiya, J. Biol. Chem., 267, pp. 20921-20926, (1992)

[5]

Tominaga, Sugie, Hirata, Morii, Fukata, Uchida, Imura, Narumiya, J. Cell Biol., 120, pp. 1529-1537, (1993)

[6]

Stasia, Jouan, Bourmeyster, Bouquet, Vignais, Biochem. Biophys. Res. Commun., 180, pp. 615-622, (1991)

[7]

Nishiyama, Sasaki, Takaishi, Kato, Yaku, Araki, Matsuura, Takai, Mol. Cell. Biol., 14, pp. 2447-2456, (1994)

[8]

Yamamoto, Marui, Sakai, Morii, Kozaki, Ikai, Imamura, Narumiya, Oncogene, 8, pp. 1449-1455, (1993)

[9]

Jalink, van Corven, Hengeveld, Morii, Narumiya, Moolenaar, J. Cell Biol., 126, pp. 801-810, (1994)

[10]

Hirata, Kikuchi, Sasaki, Kuroda, Kaibuchi, Matsuura, Seki, Saida, Takai, J. Biol. Chem., 267, pp. 20921-20926, (1992)

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