THE SMALL GTPASE RAB5 FUNCTIONS AS A REGULATORY FACTOR IN THE EARLY ENDOCYTIC PATHWAY

被引：1206

作者：

BUCCI, C ^{[1
]}

PARTON, RG ^{[1
]}

MATHER, IH ^{[1
]}

STUNNENBERG, H ^{[1
]}

SIMONS, K ^{[1
]}

HOFLACK, B ^{[1
]}

ZERIAL, M ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT ANIM SCI,COLL PK,MD 20742

来源：

CELL | 1992年 / 70卷 / 05期

关键词：

D O I：

10.1016/0092-8674(92)90306-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the in vivo functional role of rab5, a small GTPase associated with the plasma membrane and early endosomes. Wild-type rab5 or rab5ile133, a mutant protein defective in GTP binding, was overexpressed in baby hamster kidney cells. In cells expressing the rab5ile133 protein, the rate of endocytosis was decreased by 50% compared with normal, while the rate of recycling was not significantly affected. The morphology of early endosomes was also drastically changed by the mutant protein, which induced accumulation of small tubules and vesicles at the periphery of the cell. Surprisingly, overexpression of wild-type rab5 accelerated the uptake of endocytic markers and led to the appearance of atypically large early endosomes. We conclude that rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

引用

页码：715 / 728

页数：14

共 70 条

[1] ESTIMATION OF SURFACE-AREA FROM VERTICAL SECTIONS [J].