3 DIFFERENT HUMAN-TUMOR CELL-LINES CONTAIN DIFFERENT ONCOGENES

被引：227

作者：

MURRAY, MJ ^{[1
]}

SHILO, BZ ^{[1
]}

SHIH, C ^{[1
]}

COWING, D ^{[1
]}

HSU, HW ^{[1
]}

WEINBERG, RA ^{[1
]}

机构：

[1] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

来源：

CELL | 1981年 / 25卷 / 02期

关键词：

D O I：

10.1016/0092-8674(81)90054-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Foci of transformed mouse cells after transfection of human DNA from colon and bladder carcinoma cell lines [colon carcinoma SW480, bladder carcinoma] and a promyelocytic leukemia cell line [HL-60] were obtained. These foci contained a large number of human DNA sequences shown by use of highly repetitive human DNA sequence probes. Cell DNA from primary foci can be used in a subsequent cycle of transfection resulting in secondary foci that contain relatively little human DNA. Secondary foci appear to contain only the human sequences proximal to those responsible for the transformed phenotype. A set of characteristic DNA restriction fragments is found in common among secondary foci derived from each tumor cell line DNA. Comparison of the common DNA fragments found in secondary foci derived from 3 different human tumor cell lines indicates that these 3 cell lines contain 3 different transforming genes.

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页码：355 / 361

页数：7

相关论文

共 32 条

[1] DEFINED SUB-GENOMIC FRAGMENT OF INVITRO SYNTHESIZED MOLONEY SARCOMA-VIRUS DNA CAN INDUCE CELL TRANSFORMATION UPON TRANSFECTION [J].

ANDERSSON, P ;

GOLDFARB, MP ;

WEINBERG, RA .

CELL, 1979, 16 (01) :63-75

[2] CONTINUOUS GROWTH AND DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC-CELLS IN SUSPENSION CULTURE [J].

COLLINS, SJ ;

GALLO, RC ;

GALLAGHER, RE .

NATURE, 1977, 270 (5635) :347-349

[3] TRANSFORMING ACTIVITY OF DNA OF CHEMICALLY TRANSFORMED AND NORMAL-CELLS [J].

COOPER, GM ;

OKENQUIST, S ;

SILVERMAN, L .

NATURE, 1980, 284 (5755) :418-421

[4]

Fogh J., 1975, HUMAN TUMOR CELLS IN, P115, DOI [DOI 10.1007/978-1-4757-1647-45, DOI 10.1007/978-1-4757-1647-4_5]

[5] TRANSFORMATION OF THE GENE FOR HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE [J].

GRAF, LH ;

URLAUB, G ;

CHASIN, LA .

SOMATIC CELL GENETICS, 1979, 5 (06) :1031-1044

[6] GENETICS OF SOMATIC MAMMALIAN-CELLS .29. ISOLATION AND LOCALIZATION OF DNA SEGMENTS FROM SPECIFIC HUMAN-CHROMOSOMES [J].

GUSELLA, JF ;

KEYS, C ;

VARSANYIBREINER, A ;

KAO, FT ;

JONES, C ;

PUCK, TT ;

HOUSMAN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (05) :2829-2833

[7] THE CHINESE-HAMSTER ALU-EQUIVALENT SEQUENCE - A CONSERVED, HIGHLY REPETITIOUS, INTERSPERSED DEOXYRIBONUCLEIC-ACID SEQUENCE IN MAMMALS HAS A STRUCTURE SUGGESTIVE OF A TRANSPOSABLE ELEMENT [J].

HAYNES, SR ;

TOOMEY, TP ;

LEINWAND, L ;

JELINEK, WR .

MOLECULAR AND CELLULAR BIOLOGY, 1981, 1 (07) :573-583

[8] UBIQUITOUS FAMILY OF REPEATED DNA SEQUENCES IN THE HUMAN GENOME [J].

HOUCK, CM ;

RINEHART, FP ;

SCHMID, CW .

JOURNAL OF MOLECULAR BIOLOGY, 1979, 132 (03) :289-306

[9] UBIQUITOUS, INTERSPERSED REPEATED SEQUENCES IN MAMMALIAN GENOMES [J].

JELINEK, WR ;

TOOMEY, TP ;

LEINWAND, L ;

DUNCAN, CH ;

BIRO, PA ;

CHOUDARY, PV ;

WEISSMAN, SM ;

RUBIN, CM ;

HOUCK, CM ;

DEININGER, PL ;

SCHMID, CW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1398-1402

[10]

Klein G, 1980, VIRAL ONCOLOGY

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