SYNTHESIS AND ENZYMATIC PROPERTIES OF DEOXYRIBOOLIGONUCLEOTIDES CONTAINING METHYL AND PHENYLPHOSPHONATE LINKAGES

被引：81

作者：

AGARWAL, KL ^{[1
]}

RIFTINA, F ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT THEORET BIOL,CHICAGO,IL 60637

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/6.9.3009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical methods for the synthesis of short deoxyribooligonucleotides containing methyl and phenylphosphonodiester linkages have been developed. The interaction of two such nonionic dinucleotide analogs, T(pCH3)T and T(pC6H5)T, with several enzymes has been investigated. Because of the phosphonate linkage each dinucleotide exists as a diastereomeric pair as shown by thin layer chromatography and enzymatic studies. Both isomers of each dinucleotide can be phosphorylated by T4-polynucleotide kinase in the presence of [γ-32P]ATP. Only one of the diastereoisomers of each dinucleotide is slowly hydrolyzed by snake venom phosphodiesterase and acts as an inhibitor of the enzyme-catalyzed hydrolysis of 5′-labeled oligothymidylic acid. Both isomers of each dinucleotide analog are completely resistant to hydrolysis by spleen phosphodiesterase. © 1979 Information Retrieval Limited.

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页码：3009 / 3024

页数：16

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