ENTAMOEBA-HISTOLYTICA - CORRELATION OF THE CYTOPATHIC EFFECT OF VIRULENT TROPHOZOITES WITH SECRETION OF A CYSTEINE PROTEINASE

被引：93

作者：

KEENE, WE

HIDALGO, ME

OROZCO, E

MCKERROW, JH

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PATHOL,BOX 0506,SAN FRANCISCO,CA 94143

[2] INST POLITECN NACL,CTR INVEST ESTUDIOS AVANZADOS,DEPT GENET & MOLEC BIOL,MEXICO CITY 07000,DF,MEXICO

[3] UNIV CALIF BERKELEY,SCH PUBL HLTH,DEPT BIOMED & ENVIRONM HLTH SCI,BERKELEY,CA 94720

来源：

EXPERIMENTAL PARASITOLOGY | 1990年 / 71卷 / 02期

关键词：

Entamoeba histolytica; Proteinase; Virulence;

D O I：

10.1016/0014-4894(90)90022-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Work from several laboratories suggests a correlation between expression of cysteine proteinase activity and the cytopathic effect of virulent HM1 strain Entamoeba histolytica trophozoites on cultured cell monolayers. Consistent with this relationship, we find that L-6 trophozoites, mutants cloned from the HM1 parent strain, are deficient in both proteinase expression and cytopathic effect. Three other clones, with proteinase expression equal to or greater than that of the HM1 strain, express the cytopathic effect. Furthermore, a nontoxic specific proteinase inhibitor, Z-phenylalanyl-alanyl-CH2F, inhibits the cytopathic effect of live trophozoites in a dose-dependent manner. These results support the hypothesis that expression and release of the cysteine proteinase is an important factor in producing the cytopathic effect, presumably by its degradation of cell anchoring proteins. © 1990.

引用

页码：199 / 206

页数：8

共 32 条

[1]

ADAMS DO, 1980, J IMMUNOL, V124, P286

[2]

BOUVIER J, 1990, IN PRESS ARCH INVEST

[3]

de la Torre M., 1971, ARCH INVEST MED S1, V2, P165

[4] NEW MEDIUM FOR AXENIC CULTIVATION OF ENTAMOEBA-HISTOLYTICA AND OTHER ENTAMOEBA [J].