CLONING, EXPRESSION AND PARTIAL CHARACTERIZATION OF A NOVEL RAT PHOSPHOLIPASE A(2)

被引：50

作者：

CHEN, J

ENGLE, SJ

SEILHAMER, JJ

TISCHFIELD, JA

机构：

[1] INDIANA UNIV, SCH MED, DEPT MED & MOLEC GENET, INDIANAPOLIS, IN 46202 USA

[2] INCYTE PHARMACEUT INC, PALO ALTO, CA USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1215卷 / 1-2期

关键词：

PHOSPHOLIPASE A(2); CA2+ DEPENDENT; HEART; CLONING;

D O I：

10.1016/0005-2760(94)90099-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of a novel rat cDNA encoding a Ca2+-dependent, low molecular weight phospholipase A(2) (PLA(2)). A rat RNA blot hybridized with the cDNA exhibited a putative 2.4 kb transcript in heart. When the cDNA was expressed in human 293s cells, PLA, activity accumulated in the culture medium. This conditioned medium optimally hydrolyzed the phospholipids of [1-C-14] oleate-labeled Escherichia coli at neutral to alkaline pH with 10 mM or greater Ca2+. When single substrates were tested, L-alpha-palmitoyl-2-oleoyl phosphatidylcholine was more efficiently hydrolyzed than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-parmitoyl-2-arachidonyl phosphatidylethanolamine or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol.

引用

页码：115 / 120

页数：6

共 21 条

[1]

CAO YZ, 1987, J BIOL CHEM, V262, P16927

[2]

CHEN J, 1994, J BIOL CHEM, V269, P2365

[3]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[4] EVOLUTIONARY RELATIONSHIPS AND IMPLICATIONS FOR THE REGULATION OF PHOSPHOLIPASE-A2 FROM SNAKE-VENOM TO HUMAN SECRETED FORMS [J].