STUDIES ON PHOSPHATIDYLCHOLINE VESICLES . FORMATION AND PHYSICAL CHARACTERISTICS

被引：1526

作者：

HUANG, CH

机构：

[1] Department of Biochemistry, University of Virginia, School of Medicine, Charlottesville

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 01期

关键词：

D O I：

10.1021/bi00829a048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the separation of phosphatidylcholine vesicles, formed by prolonged ultrasonic irradiation under nitrogen in 0.1 M buffered NaCl solution, by molecular sieve chromatography on large-pore agarose gels. One fraction of the separated vesicles was found to be homogeneous by the criteria of gel filtration, sedimentation velocity ultracentrifu-gation, and electron microscopy. Experimental data which support the homogeneous vesicle as a shelllike structure, about 250 A in diameter, with a continuous phosphatidylcholine bilayer surrounding a volume of solvent are presented. The following physical parameters were established for the homogeneous vesicles: s20,w, 2.1 S; D20,w, 1.87 × 10-7cm2sec-1; ̅v, 0.9885 ml g-1; [η], 0.041 dl g-1; and vesicle weight, 2.1 × 106. © 1969, American Chemical Society. All rights reserved.

引用

页码：344 / +

页数：1

共 45 条

[1]

ACKERS GK, 1967, J BIOL CHEM, V242, P3237

[2]

ALEXANDER P, 1961, ANALYTICAL METHOD ED, V3, P58

[3] DIFFUSION OF UNIVALENT IONS ACROSS LAMELLAE OF SWOLLEN PHOSPHOLIPIDS [J].