HIGHLY SENSITIVE AND REPRODUCIBLE MICROCYTOTOXICITY ASSAY FOR DEMONSTRATING CYTOTOXIC ANTIBODIES TO CELL-SURFACE ANTIGENS

被引：8

作者：

HELLSTROM, I

BROWN, JP

KLITZMAN, JM

HELLSTROM, KE

机构：

[1] UNIV WASHINGTON,SCH MED,DEPT MICROBIOL IMMUNOL,SEATTLE,WA 98195

[2] UNIV WASHINGTON,SCH MED,DEPT PATHOL,SEATTLE,WA 98195

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1978年 / 22卷 / 3-4期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0022-1759(78)90044-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay is described, in which target cells are suspended in diluted rabbit serum (as a source of complement) and pipetted into the wells of Falcon No. 3034 Microtest plates, to which diluted antiserum (or control serum) has been added. After 18-24 h of incubation, the plates are stained with crystal violet and counted using an electronic Quantimet image analyzer. This technique could reproducibly detect complement-dependent cytotoxic antibodies to target cell H-2 antigens and to MuLV gp70 on cell lines which expressed this antigen, at serum dilutions up to 1:160,000. The cytotoxic antibodies could be removed by absorption of sera with cells carrying the respective antigens. © 1978.

引用

页码：369 / 381

页数：13

共 11 条

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[2]

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[3]

DeLeo, Shiku, Takahashi, John, Old, J. Exp. Med., 146, (1977)

[4]

Elder, Jensen, Bryant, Lerner, Nature, 267, (1977)

[5]

Gorer, O'Gorman, Transplant. Bull., 3, (1956)

[6]

Hellstrom, Hellstrom, In Vitro Methods in Cell-mediated Immunity, (1971)

[7]

Hellstrom, Sjogren, Journal of Experimental Medicine, 125, (1967)

[8]

Hellstrom, Hellstrom, Pierce, International Journal of Cancer, 3, (1968)

[9]

Sanderson, Nature, 204, (1964)

[10]

Takasugi, Klein, Transplantation, 9, (1970)

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