ROUTES TO ACTIVE PROTEINS FROM TRANSFORMED MICROORGANISMS

被引：31

作者：

BUCHNER, J

RUDOLPH, R

机构：

[1] UNIV REGENSBURG, INST BIOPHYS & BIOCHEM, W-8400 REGENSBURG, GERMANY

[2] BOEHRINGER MANNHEIM GMBH, CTR BIOCHEM RES, W-8122 PENZBERG, GERMANY

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1991年 / 2卷 / 04期

关键词：

D O I：

10.1016/0958-1669(91)90077-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over-expression of recombinant proteins in microbial hosts results in the formation of active soluble protein or of insoluble aggregates (inclusion bodies). Efficient in vitro refolding strategies have been developed to reactive inactive proteins from inclusion bodies. Co-expression of molecular chaperones may provide a tool to promote correct structure formation of recombinant proteins in vivo.

引用

收藏

页码：532 / 538

页数：7

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